UNIVERSAL VARIABLE FRAGMENTS

摘要

A method and associated kit for analysing genomic DNA in a sample, said method comprising: providing first and second oligonucleotide primers, said first oligonucleotide primer being a 5' variation generator, and comprising a repeat sequence and at least one nucleotide inconsistent with the repeat pattern, said first oligonucleotide primer having at least one nucleotide positioned on the first oligonucleotide's 5' end, and said second oligonucleotide primer being a 3' fragment generator starting within such a genetic distance that amplification of the genomic DNA can be performed; conducting a nucleic acid amplification on said genomic DNA in the sample at a relatively low annealing temperature using both the first and second oligonucleotide primers, said amplification being conducted under conditions such that neither the first nor the second oligonucleotide primer alone can amplify DNA, thus producing DNA fragments based on repeat sequences on one end of the genomic DNA and other sequences based on the opposite end of the genomic DNA; and analysing the amplified products thus produced to determine the length of a repeat sequence found in said genomic DNA.