发明名称 |
MULTIPLE GENE EXPRESSION FOR ENGINEERING NOVEL PATHWAYS AND HYPEREXPRESSION OF FOREIGN PROTEINS IN PLANTS |
摘要 |
Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system.
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申请公布号 |
WO0164024(A1) |
申请公布日期 |
2001.09.07 |
申请号 |
WO2001US06276 |
申请日期 |
2001.02.28 |
申请人 |
AUBURN UNIVERSITY;UNIVERSITY OF CENTRAL FLORIDA;DANIELL, HENRY;MOAR, WILLIAM |
发明人 |
DANIELL, HENRY;MOAR, WILLIAM |
分类号 |
C07K14/325;C12N15/79;C12N15/82;(IPC1-7):A01H5/02;A01H5/10;A01H13/00;C12N1/20;C12N1/12;C12N5/04;C12N15/29;C12N15/32;C12N15/52;C12N15/31;C12N15/17;C12N15/13;C12N15/63;C12N15/74 |
主分类号 |
C07K14/325 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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