DNA JOINING METHOD

摘要

The present invention provides a method to directionally clone any template DNA molecule into a single restriction site of any vector. The vector ends may b e generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join two or more linear DNA molecules sharing ends with appropriate complementation.