发明名称 |
Procedure for the production of ethanol from lignocellulosic biomass using a heat-tolerant yeast |
摘要 |
<p>It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230 DEG C for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of beta -glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42 DEG C. <IMAGE></p> |
申请公布号 |
EP1130085(A1) |
申请公布日期 |
2001.09.05 |
申请号 |
EP20010500028 |
申请日期 |
2001.02.01 |
申请人 |
CENTRO DE INVESTIGACIONES ENERGETICAS MEDIOAMBIENTALES Y TECNOLOGICAS (C.I.E.M.A.T.) |
发明人 |
BALLESTEROS PERDICES, IGNACIO;BALLESTEROS PERDICES, MERCEDES;OLIVA DOMINGUEZ, JOSE MIGUEL;CARRASCO GARCIA, JUAN ESTEBAN |
分类号 |
C12P7/10;(IPC1-7):C12N1/16;C12N9/42;C12N1/22 |
主分类号 |
C12P7/10 |
代理机构 |
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代理人 |
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地址 |
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