Modified lipolytic enzymes and their use

摘要

There is provided an amino acid sequence encoding a lipase, which has at least 65% sequence homology with the amino acid seqence of a Candida ruginosa lipase having the amino acid sequence substantially as depicted in Figure 1 and SEQ ID NO:2, and which differs by at least one amino acid substitution at a selected site in said lipase. Also provided is a modified nucleic acid sequence encoding a lipase variant, preferably a variant of lipase 1 wherein the modified residues are located at one or more of the positions T11 and T201 in the amino acid sequence of Candida rugosa lipase 1. Preferably, the threonine at one or more of the positions 11 and 201 is replaced by alanine. Furthermore, the use of the lipase variants and/or amino acid sequences is provided in a manner known per se, in particular in a process requiring high specificity towards C16-C18 acyl chains.