| 摘要 |
<p>Described is a method for detecting the presence of at least one single stranded target nucleic acid in a sample comprising providing said sample or sub-sample thereof, with a first nucleic acid probe complementary to a distinct part of said target nucleic acid and a second nucleic acid probe complementary to a second part of said target nucleic acid located essentially adjacent to said distinct part of said target nucleic acid, wherein said probes further comprise a tag which is essentially non-complementary to said target nucleic acid, the method further comprising incubating said sample allowing hybridisation of complementary nucleic acids, connecting any essentially adjacent probes, amplifying any connected probe nucleic acid, wherein amplification is initiated by binding of a nucleic acid primer specific for said tag and detecting an amplicon, wherein at least one nucleic acid probe comprises enzymatic template directed polymerised nucleic acid prior to said connecting. Further, nucleic acid probes for use in and a kit for performing the said method are disclosed.</p> |
