Amplification of long nucleic acid sequences by PCR

摘要

The invention is directed to a DNA polymerase composition for the polymerase chain reaction amplification of nucleic acid sequences longer than 10 kb consisting of a combination of a first DNA polymerase and a second DNA polymerase, wherein said first DNA polymerase is Thermus thermophilus DNA polymerase, and wherein said second DNA polymerase is a thermostable DNA polymerase that increase the maximum target length amplifiable by providing both 3'-to-5' exonuclease activity and polymerase activity and wherein said DNA polymerase composition consists of about 0.8-2.5 units of first DNA polymerase for each 0.015-0.15 units of second DNA polymerase.