发明名称 |
Methods and compositions for synthesis of oligosaccharides using mutant glycosidase enzymes |
摘要 |
Mutant glycosidase enzymes are formed in which the normal nucleophilic amino acid within the active site has been changed to a non-nucleophilic amino acid. These enzymes cannot hydrolyze disaccharide products, but which can still form them. Using this enzyme, oligosaccharides are synthesized by preparing a mixture of an alpha-glycosyl fluoride and a glycoside acceptor molecule; enzymatically coupling the alpha-glycosyl fluoride to the glycoside acceptor molecule to form a glycosyl glycoside product using the mutant glycosidase enzyme; and recovering the glycosyl glycoside product. Particular enzymes include a mutant form of Agrobacterium beta-Glucosidase in which the normal glutamic acid residue at position 358 is replaced with an alanine residue.
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申请公布号 |
US6284494(B1) |
申请公布日期 |
2001.09.04 |
申请号 |
US19980091272 |
申请日期 |
1998.09.29 |
申请人 |
THE UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
WITHERS STEPHEN G.;MACKENZIE LLOYD;WANG QINGPING |
分类号 |
C12N9/24;C12N9/26;C12P19/04;(IPC1-7):C12P19/44;C12P19/12;C12N9/42 |
主分类号 |
C12N9/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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