发明名称 |
A PROCESS FOR GENERATING CYTOPLASMIC MALE STERILE LINE IN RICE AND OTHER CROPS BY RNA EDITING |
摘要 |
<p>The present invention relates to a cytoplasmic male sterility (CMS) transgen ic plant prepared by RNA editing for expressing unedited nad 9 gene disenabling ATP production in the mitochondria of plants and a process for preparing the same. The said process comprises: cloning an un-edited nad 9 gene by digesti ng the PCR product obtained from mitochondrial DNA to obtain pNG3cloning crop with un-edited nad 9 gene with the targeting sequence under ubiquitin promot er a NOS terminator to obtain pNG11 co-bombarding pNG11 constructs in a manner as herein described with hygromycin gene (pLAU6 hph construct) to generate plan ts containing un-edited nad 9 gene; analysing un-edited nad 9 (pNG11) construct with a control plant having edited nad 9 gene, pNG10to determine the presenc e of nad 9 gene.</p> |
申请公布号 |
CA2401281(A1) |
申请公布日期 |
2001.08.30 |
申请号 |
CA20012401281 |
申请日期 |
2001.02.26 |
申请人 |
AVESTHA GENGRAINE TECHNOLOGIES PVT. LTD.;GRINENBERGER, JEAN-MICHEL |
发明人 |
GOSWAMI, SULIP KUMAR;VENKATARAMIAH, MAHESH;RAYAPURAM, NAGANAND;PATELL, VILLOO MORAWALA;JOY, JOMA |
分类号 |
A01H5/00;C12N9/02;C12N15/09;C12N15/82;(IPC1-7):C12N15/82;A01H1/00;C12N15/29;C12N15/53 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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