发明名称 |
METHODS FOR CHARACTERIZING POLYMORPHISMS |
摘要 |
Methods are described for analyzing at least one polymorphic site in a biological sample containing at least one single-stranded template, including the steps of: combining the biological sample with a primer specific for each polymorphic site in each template and a primer extension preparation, to form an assay mixture; wherein the preparation includes: chain terminating nucleotides forming a first nucleotide class, chain elongating nucleotides forming a second nucleotide class such that the second nucleotide class does not include a nitrogenous base present in the first nucleotide class, and a template-dependent nucleic acid polymerase; incubating the mixture for a time and at a temperature sufficient to extend each primer by addition of at least one nucleotide; and determining the size of each extended primer. Kits suitable for practising the invention are also provided.
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申请公布号 |
WO0162966(A2) |
申请公布日期 |
2001.08.30 |
申请号 |
WO2001GB00828 |
申请日期 |
2001.02.26 |
申请人 |
GEMINI GENOMICS PLC |
发明人 |
HENRIKSSON, JAN, ANDERS, RAGNAR;JONSSON, BO, JOERGEN, ANDERS;NORBERG, LEIF, TORBJOERN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
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