| 摘要 |
The present invention relates to a process for preparing gamma-polyglutamic acid (gamma-PGA) from high-viscous culture broth, more particularly, to an economical and efficient process for preparing gamma-polyglutamic acid from high-viscous culture broth with easy removal of microorganisms and a subsequent concentrating process employing a filtration membrane. The present invention provides the process for preparing gamma-polyglutamic acid from high-viscous culture broth which comprises the steps of: culturing gamma-polyglutamic acid-producing microorganism for 15-30 hours under condition of pH 5.0-7.5 and 30-40° C. to obtain high-viscous culture broth with a concentration of 20-30 g/L; removing microorganism from the high-viscous culture broth thus obtained by adjusting pH to 2-4 or 7-9 and centrifuging at 3,000-9,000 rpm for 10-50 minutes; and, obtaining gamma-polyglutamic acid by concentrating the culture broth employing filter and precipitating gamma-polyglutamic acid by addition of alcohol. In accordance with the invention, polyglutamic acid can be prepared from high-viscous culture broth in an economical and efficient manner, which would make it possible that polyglutamic acid can be applied widely as a universal material.
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