发明名称 |
MULTIPLEX LIGATABLE PROBE AMPLIFICATION |
摘要 |
Described is an improved multiplex ligation-dependent amplification method for detecting the presence and quantification of at least one specific single stranded target nucleic acid sequence in a sample using a plurality of probe sets of at least two probes, each of which includes a target specific region and a non-complementary region comprising a primer binding site. The probes belonging to the same set are ligated together when hybridised to the target nucleic acid sequence and amplified by a suitable primer set. By using a femtomolar amount of the probes a large number of different probe sets can be used to simultaneously detect and quantify a corresponding large number of target sequences with high specificity.
|
申请公布号 |
WO0161033(A2) |
申请公布日期 |
2001.08.23 |
申请号 |
WO2001EP01739 |
申请日期 |
2001.02.15 |
申请人 |
SCHOUTEN, JOHANNES, PETRUS |
发明人 |
SCHOUTEN, JOHANNES, PETRUS |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|