BIOCHEMICAL BLOCKING LAYER FOR LIQUID CRYSTAL ASSAY

摘要

A rubbed substrate structure for use in a liquid crystal assay device, includes: a biochemical blocking compound chemically immobilized on a surfac e of one side of a support forming a biochemical blocking layer; and a biomolecule recognition agent deposited on the side of the support containin g the biochemical blocking layer. The biomolecule recognition agent includes a recognition site capable of selectively recognizing a target species to be detected by the liquid crystal assay device. Additionally, the surface on th e side of the support containing the biochemical blocking layer is rubbed such that it possesses features that drive a uniform anchoring of liquid crystals when the liquid crystals contact the side of the support containing the biochemical blocking layer. A method for preparing a rubbed substrate structure for use in a liquid crystal assay device, optical cells for use in a liquid crystal assay, liquid crystal assay devices, kits for use in a liquid crystal assay, and methods for detecting the presence of a target species using a liquid crystal assay device are also disclosed.