METHOD FOR EXTRACTING AND PURIFYING ANTICANCER COMPONENT FROM GREEN TEA

摘要

PURPOSE: Provided is a method for extracting and purifying an anticancer component, Epigallocatechin Gallate among Catechin compounds, from green tea. CONSTITUTION: The method for extracting and purifying Epigallocatechin Gallate comprises the steps of: extracting green tea leaves with pure water of 50-60 deg.C to obtain water fraction; layer-separating the water fraction by mixing it with chloroform in a volume ratio of 1:1; layer-separating the water layer obtained from previous step by mixing it with ethylacetate in a volume ratio of 1:1; purifying the ethylacetate layer obtained from the previous step with liquid chromatography; and purifying the purified extract with reversed phase liquid chromatography.