Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion

摘要

A method for discriminating between two or more alleles which differ by only a single nucleotide is provided. This method is suitable for use in solution or on a solid phase and employs the use of restriction enzyme digestion of DNA in combination with Melting Curve Analysis. Also contemplated are specific markers to be included in melting curve analysis and melting curve genotyping experiments.