| 摘要 |
<p>The present invention relates to a method for producing recombinant rotavirus structural proteins by culturing transformed plant cell, and to a vaccine composition comprising the same as an effective component. More specifically, the method comprises the steps of preparing the expression vector comprising cDNA fragment encoding rotavirus structural proteins; transforming plant cell with the expression vector; and recovering the vaccine composition including the rotavirus antigen from cell culture. The method for producing the recombinant rotavirus structural proteins has advantages in higher yield of more than 0.3 mg/L, lower cost and lower contamination hazard than those of the eukaryotic expression system such as baculovirus and animal cell, and can be used for producing edible vaccine. Also, the invention shows that the rotavirus structural protein can be produced with the high aspect rotary vessel reactor.</p> |
