| 摘要 |
<p>Described is a method of preparing recombinant nucleic acids, which comprises the steps of: a) amplification of a 1st nucleic acid fragment (x) in the presence of a polymerization agent, the various deoxynucleoside triphosphates, and two primers (x1/x2) hybridizing to form opposite strands of the nucleic acid; b) amplification of a 2nd nucleic acid fragment (y) in the presence of a polymerization agent, the various deoxynucleoside triphosphates and two primers (y1 y2) hybridizing to form opposite strands of the nu cleic acid; c) a sequence identical in both primers is additionally present between this 3' end and the 5' end of primers (x2) and (y1), which corresponds to the sequence cleaved by the restriction enzyme; d) restriction of the amplified 1st and 2nd nucleic acids with (a) restriction endonuclease(s) recognizing the recognition sequences defined in (a) and (b); e) ligation of the nucleic acid fragments obtained in (d).</p> |
