| 摘要 |
Present invention involves a recombination assay for the HIV envelope genes, <i>gp120</i>, <i>gp41</i>, and <i>gp160</i>. It further involves env-deleted proviral clones, the optimization of the PCR amplification of the corresponding <i>env</i>-genes and the subsequent sequencing of these genes. These techniques have been applied on several HIV-1(NL4.3) strains selected <i>in vitro</i> in the presence of increasing concentrations of inhibitors of HIV entry and evaluated for the phenotypic resistance of these recombined viruses. This phenotypic resistance has been correlated with genotypic resistance. Present invention also involves a recombination assay for the integrase gene. |
