发明名称 |
Direct detection and mutation analysis of low copy number nucleic acids |
摘要 |
A direct detection and quantitation of RNA or DNA contained in a sample is obtained by capillary electrophoresis of the RNA or DNA hybridized to a DNA or RNA probe of complementary sequence stabilized by the combination of a fluorophore terminally conjugated to the probe and a dye intercalating the RNA-DNA or DNA-DNA hybrid so formed. The RNA or DNA is quantified by measuring the total fluorescence emitted by the electrophoresed hybrid upon excitation by a laser generated light beam. |
申请公布号 |
AU3477501(A) |
申请公布日期 |
2001.08.14 |
申请号 |
AU20010034775 |
申请日期 |
2001.02.02 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION. |
发明人 |
JILL M. KOLESAR |
分类号 |
C12Q1/68;G01N27/447 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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