| 摘要 |
<p>Vitamin B12 is efficiently produced at a large content and a high yield by acclimatizing a mesophilic methane bacterium obtained from digested sludge in an H2/CO2 medium; growing the thus acclimatized methane bacterium on a support containing inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor; allowing the bacterium to metabolize a mixed gas, which is prepared by adding hydrogen to at least one of coal gas and biogas, into methane; and, at the same time, recovering cobalamin contained in the fermented liquor as an extracellular product in the form of cyanocobalamin by using potassium cyanide.</p> |
