Determining extracellular ion concentrations, useful e.g. for pharmaceutical screening, in the basolateral regions of adherent cells, using immobilized fluorescent dyes

摘要

Method for determining extracellular ion concentrations, and their changes, in the basolateral region of adherent cells. The cells are deposited on a matrix that contains an ion-specific, immobilized fluorescent dye (I) and is present on a transparent substrate. The ion concentration is then determined by fluorescence measurements. An independent claim is also included for a device for the new method comprising a transparent substrate with, deposited on it, a matrix containing (I).