发明名称 |
CELL CULTURE PROCESS FOR GLYCOPROTEINS |
摘要 |
A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding said glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt and prefers and octahedral coordination geometry, and/or (c) a plasma component. In this process, the occupancy of an N-linked glycosylation site occupied only in a fraction of a glycoprotein is enhanced. Such culturing is preferably carried out at a temperature of between about 30 DEG C and 35 DEG C and/or in the presence of up to about 2mM of a butyrate salt and/or in the presence of a cell-cycle inhibitor. |
申请公布号 |
WO0065070(A9) |
申请公布日期 |
2001.08.02 |
申请号 |
WO2000US10810 |
申请日期 |
2000.04.21 |
申请人 |
GENENTECH, INC. |
发明人 |
ANDERSEN, DANA, C.;BRIDGES, TIFFANY, M.;GAWLITZEK, MARTIN;HOY, CYNTHIA, A. |
分类号 |
C12N9/64;C12N5/10;C12N9/72;C12N15/58;C12P21/00;C12P21/04;C12R1/91 |
主分类号 |
C12N9/64 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|