USE OF A NUCLEOTIDE SEQUENCE FOR ENHANCING PROTEIN SYNTHESISAND EXPRESSION OF PROTEINS

摘要

The present invention is related to the use of nucleotide sequences substantially similar to the cDNA sequence (SEQ ID NO:2:) obtainable from th e leader sequence (SEQ ID NO:1:) of the Cocksfoot mottle virus (CfMV) which is capable of enhancing protein synthesis and expression of proteins, especiall y in plants such as cereals. Also disclosed is a method for producing potentia l enhancer elements by selecting 5'UTRs having a capacity of producing hairpin loop structures and preparing substantially similar nucleic acid sequences. In addition a method for enhancing the expression in plants as well as the properties characteristic for the nucleotide sequence which are responsible for the enhanced expression.