| 摘要 |
PROBLEM TO BE SOLVED: To obtain a reagent, a method and a kit which are used for determining the expression of hTERT mRNA encoding active hTERT protein and improved more than already described method by permitting more exact estimation of telomerase activity. SOLUTION: This method is a method for determining telomerase activity in a human sample which comprises (a) acting an RNA derived from the human sample on an amplifying reagent comprising a primer pair (here, the above primer pair comprises the first primer complementary to one side of chain in a specific sequence of double strand hERT gene sequence present in a specific exon domain and the second primer complementary or substantially complementary to the other side of chain in the above hTERT gene sequence present in the specific exon domain), (b) carrying out an amplifying reaction, (c) measuring the formation of amplified products and (d) determining the amount of hTERT mRNA contained in the above sample derived from the result obtained on the process (c). The kit for determining hTERT mRNA contained in the human sample is used to detect the presence of the β domain of the hTERT mRNA for diagnosing and prognosing cancer. |
