| 摘要 |
A cell and tissue culture device comprises a closed pressurization circuit (2) able to deliver a selected gas under a selected pressure, at least a culture chamber (6) accommodating means (13) for supporting at least a deformable membrane (14) forming in chamber (6) an interface between first (17) and second (16) portions with variable complementary volumes, wherein the first portion (17) is configured for receiving cells and/or tissues to be grown for being supplied with culture fluid by a first tank (8), and said second portion (16) is configured for being supplied with gas by the pressurization circuit (2) and for discharging at least a portion of this gas. In addition, it comprises control means (3,4) capable of determining, according to selected criteria relating to the type of culture to be achieved, the gas to be fed into the second portion (16), its pressure and the time for feeding in this gas, and of controlling access to the first and second portions of the chamber (6) so as to control together the shape of the membrane (14), the culture fluid supply and the flow of this fluid in the first portion. |
