发明名称 |
REGENERATION AND GENETIC TRANSFORMATION OF ACACIA MANGIUM |
摘要 |
The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in th e regenerated plants and Southern blot hybridization prove the incorporation o f the foreign gene into the host genome and expression of the foreign gene.</S DOAB>
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申请公布号 |
CA2362767(A1) |
申请公布日期 |
2001.07.26 |
申请号 |
CA20002362767 |
申请日期 |
2000.01.19 |
申请人 |
INSTITUTE OF MOLECULAR AGROBIOLOGY |
发明人 |
HONG, YAN;XIE, DEYU |
分类号 |
A01H1/00;A01H4/00;C12N15/82;C12N15/84;(IPC1-7):C12N15/29;A01H5/00 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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