METHOD FOR PREPARING STABLE AND FUNCTIONALISED COLLOIDAL PARTICLES AND RESULTING PARTICULATE REAGENT

摘要

The invention concerns a method for preparing functionalised stable particles which consists in: (i) providing a colloidal dispersion of organic and/or inorganic particles in an aqueous medium, said particles having functional groups X capable of interacting with other functional groups, said functional groups X being selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof; (ii) contacting said dispersion with a dissolved polymer bearing functional groups, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, alpha -halogenocarbonyl, sulphonic acid, isocyanate and isothiocyanate groups to form a mixture; and (iii) incubating said mixture in predetermined conditions of temperature, pH and incubation time, so that at least a fraction of said functional groups X reacts with at least a fraction of the functional groups Z and/or Z' to form covalent bonds. The invention also concerns a particulate reagent having affinity for biological compounds.