摘要 |
PURPOSE: Provided is a novel plasmid which is suitable for a plasmid vector having improved characteristics in Coryneform bacteria which produce amino acids, and nucleotides. CONSTITUTION: The mutually compatible plasmids pTET3 and pCRY4, are isolated from Corynebacterium glutamicum DSM 5816. pTET3 contains about 27.8 kbp, and includes a replication region of 4539 bp and a 7136 bp antibiotic resistance region containing the tetA gene (resistance to tetracycline) and the aadA gene (resistance to streptomycin and spectinomycin. pCRY4 contains about 48 kbp and includes a replication region of 1856 bp.
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