Potassium channel mutants of the saccharomyces cerevisiae yeast and the use thereof for the screening of eukaryotic potassium channels

摘要

Identifying inhibitors or activators (A) of a eukaryotic potassium channel (KC) by applying a test compound to a mutant Saccharomyces cerevisiae cell in which: (i) the three endogenous KC (TRK1, TRK2 and TOK1) are not expressed; but (ii) a eukaryotic KC is expressed heterologously, where the effect of the compound on the eukaryotic KC is then determined. Independent claims are also included for the following: (1) mutant S. cerevisiae cells in which the three endogenous KC are not expressed; (2) preparing cells of (1) by knockout destruction of the relevant genes; (3) similar method for identifying activators of eukaryotic KC in which the cells are treated with both a test compound and a known inhibitor of KC; (4) test kits containing the cells of (1); and (5) preparation of pharmaceuticals using (A) identified by the new methods. - ACTIVITY : None given. - MECHANISM OF ACTION : Potassium channel activator or inhibitor..