发明名称 |
PROCEDE D'IMMOBILISATION DE REACTIF(S) AFFIN(S) SUR PHASE SOLIDE HYDROPHOBE |
摘要 |
The invention relates to a method for the immobilisation of (an) affinity reagent(s) on a hydrophobic solid phase and can be used in biological assays for analyte detection. In particular, the invention relates to a method of immobilising an affinity reagent on a hydrophobic solid phase with a carboxylic functionality, comprising a solid phase activation step and a solid phase and affinity reagent coupling step. Said method is characterised in that a combination of a carbodiimide and a phosphate buffer is used for the activation step in the presence of a co-activator and in an acid medium and that the coupling step is carried out in a basic medium. The invention further relates to the reactive complexes obtained by said method and their use in immunoassay kits, hybridisation kits and enzymatic test kits.
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申请公布号 |
FR2803913(A1) |
申请公布日期 |
2001.07.20 |
申请号 |
FR20000000376 |
申请日期 |
2000.01.13 |
申请人 |
PASTEUR SANOFI DIAGNOSTICS |
发明人 |
BABIN FABIENNE;HAMON LAURENCE;RIEUNIER FRANCOIS |
分类号 |
G01N33/531;C12N11/02;G01N33/53;G01N33/543;G01N33/566;(IPC1-7):G01N33/544;C12Q1/00;C12N11/00;B01J20/32 |
主分类号 |
G01N33/531 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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