METHOD FOR IMMOBILISATION OF (AN) AFFINITY REAGENT(S) ON A HYDROPHOBIC SOLID PHASE

摘要

The invention relates to a method for the immobilisation of (an) affinity reagent(s) on a hydrophobic solid phase and can be used in biological assays for analyte detection. In particular, the invention relates to a method of immobilising an affinity reagent on a hydrophobic solid phase with a carboxylic functionality, comprising a solid phase activation step and a solid phase and affinity reagent coupling step. Said method is characterised in that a combination of a carbodiimide and a phosphate buffer is used for the activation step in the presence of a co-activator and in an acid medium and that the coupling step is carried out in a basic medium. The invention further relates to the reactive complexes obtained by said method and their use in immunoassay kits, hybridisation kits and enzymatic test kits.