METHOD FOR INCREASING SURVIVAL RATE OF CELLS IN ANIMAL CELL CULTURE UNDER HYPOXIA CONDITION

摘要

The present invention relates to a method for increasing survival rate of cells in animal cell culture under hypoxia condition by adding antibiotics to the culture media. The method of present invention comprises a step of culturing animal cells in culture media containing antibacterial agent of quinolones, quinones, aminoglycosides or chloramphenicol at the concentration range of 0.1 to 1000 mu g/ml. The invented method can be practically applied for high-density animal cell culture to produce recombinant proteins or cultured cells.