发明名称 |
PRODUCTION OF RECOMBINANT ORGANISMS |
摘要 |
A method comprising: a) altering a chromosomal sequence of a donor nucleus of a donor cell by introducing a pair of single-stranded targeting polynucleotides, and a recombinase into said donor nucleus of said donor cell, wherein said pair of targeting polynucleotides are substantially complementary to each other and each comprising a homology clamp that substantially corresponds to or is substantially complementary to a predetermined DNA sequence of said nucleus; and, b) transplanting said nucleus into an oocyte to produce a recombinant zygote. A method of altering a nucleic acid sequence of a mitochondria or chloroplast of a cell comprising: introducing into a cell a pair of single-stranded targeting polynucleotides, and recombinase, wherein said pair of targeting polynucleotides are substantially complementary to each other, and each comprising a homology clamp that substantially corresponds to or is substantially complementary to a predetermined nucleic acid sequence of said mitochondria or chloroplast, whereby said sequence is altered.
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申请公布号 |
WO0150847(A2) |
申请公布日期 |
2001.07.19 |
申请号 |
WO2000US34933 |
申请日期 |
2000.12.21 |
申请人 |
PANGENE CORPORATION |
发明人 |
SARGENT, ROY, GEOFFREY;VALLERGA, ANNE, KATHRYN;PATI, SUSHMA;ZARLING, DAVID, A. |
分类号 |
C12N9/10;C12N15/10;C12N15/82;C12N15/873;C12N15/90;(IPC1-7):A01K67/027 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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