发明名称 |
Nucleotidesequences encoding Pck |
摘要 |
Isolated polynucleotide (I) from coryneform bacteria comprises a sequence at least 70% identical to the sequence that encodes a fully defined 610 amino acid protein (II) sequence (S2) given in the specification, sequences in plasmids pEK-pckA and pEK-pckB, a sequence encoding a polypeptide at least 70% identical to (II), their complements or a sequence of at least 15 consecutive bases from (I). Isolated polynucleotide (I) from coryneform bacteria comprises: (a) a sequence at least 70% identical to a sequence that encodes the fully defined 610 amino acid sequence (S2) given in the specification; (b) a sequence at least 70% identical to a sequence that encodes (S2) and is present in plasmids pEK-pckA and pEK -pckB; (c) a sequence encoding a polypeptide with an amino acid sequence at least 70% identical to (S2); (d) the complements of (a), (b) or (c); (e) a sequence of at least 15 consecutive bases from (a), (b), (c) or (d) Independent claims are also included for the following: (1) vectors (III) pEK-pckA and pEK-pckB (2) vector (IV) pK19mobsacB DELTA pck (deposited in Escherichia coli DH5 alpha as DSM 13047); corynebacteria comprising (III) or (IV) or including a DELTA pck deletion; and (3) fermentative production (P) of L-amino acids using a bacterium in which either (I) is downregulated or the activity of its encoded protein is reduced. |
申请公布号 |
EP1094111(A3) |
申请公布日期 |
2001.07.18 |
申请号 |
EP20000121715 |
申请日期 |
2000.10.05 |
申请人 |
DEGUSSA AG;FORSCHUNGSZENTRUM JUELICH GMBH |
发明人 |
EIKMANNS, BERNHARD, PROF. DR.;RIEDEL, CHRISTIAN;SAHM, HERMANN, PROF.;MOECKEL, BETTINA, DR. |
分类号 |
C12N15/09;C12N1/21;C12N9/88;C12N15/00;C12N15/53;C12N15/54;C12N15/60;C12P13/08;C12R1/15 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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