发明名称 |
Detection of nucleic acids by strand displacement |
摘要 |
Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target. In the presence of target, however, the second oligonucleotide(s) of the detector nucleic acid is/are completely or partially displaced from the first, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence which may be detected as an indication of the presence of the target sequence.
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申请公布号 |
US6261784(B1) |
申请公布日期 |
2001.07.17 |
申请号 |
US20000599164 |
申请日期 |
2000.06.22 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
NADEAU JAMES G.;HSIEH HELEN V.;PITNER J. BRUCE;LINN C. PRESTON |
分类号 |
G01N21/64;C07H21/00;C12N15/09;C12Q1/68;G01N33/542;G01N33/566;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
G01N21/64 |
代理机构 |
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主权项 |
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