| 摘要 |
A DNA polymerase having an amino acid sequence comprising substantially the same amino acid sequence as that of Thermus aquaticus or Thermus flavus DNA polymerase, excluding the N-terminal 280 ami no acid residues of Thermus aquaticus DNA polymerase or the N-terminal 279 amino acid residues of Thermus flavus DNA polymerase, rec ombinant DNA sequences encoding said DNA polymerases, vectors comprising said DNA sequences, and host cells containing such vector s. A formulation of thermostable or other DNA polymerase s comprising a majority component comprised of at least one thermostable or ot her DNA polymerase of the type described above, wherein the DNA polymerase lacks 3'-exonuclease activity, and a minority component c omprised of at least one thermostable DNA polymerase exhibiting 3'-exonuclease activity, and an improved method for enzymatic ext ension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein t he above formulation is made and used to catalyse primer extension, are also provided. |
