MOBILITY-MODIFYING CYANINE DYES

摘要

The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the heterocyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activit y. The ability to predictably tune the relative electrophoretic mobilities of t he dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence- based 4-color nucleic acid sequencing reactions.