TH1 SPECIFIC CD4 T CELL LINES AND METHOD FOR INDUCING THEM <i>EX VIVO</i>

摘要

A TH1 specific CD4 T cell line, inducing an efficient CD8 response against an infection caused by an infectious agent, is obtained by removing from a donor a biological sample containing T cells; isolating the CD4<+> T cells from said sample; simultaneously providing dendritic cells isolated from the same sample or another sample derived from the same donor; subjecting the previously isolated CD4<+> T cells to in vitro immunisation with a peptide of a protein of the infectious agent exhibiting at least a T epitope, in the presence of the previously obtained dendritic cells; performing at least a restimulation, in the same conditions as for immunisation, optionally substituting the dendritic cells with B cells from the same donor.