发明名称 |
NON-CORROSIVE COMPOSITION AND METHODS USEFUL FOR THE EXTRACTION OF NUCLEIC ACIDS |
摘要 |
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
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申请公布号 |
CA2210012(C) |
申请公布日期 |
2001.07.10 |
申请号 |
CA19912210012 |
申请日期 |
1991.07.12 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
MEYER, RICH B., JR.;CIMLER, B. MELINA;VAN NESS, JEFFREY;VERMUELEN, NICHOLAAS MARTHINUS JOHANNES |
分类号 |
C12N15/10;C12Q1/68;G01N1/28;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/10 |
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