cDNA and genomic clones encoding human mu opiate receptor and the purified gene product

摘要

A human mu opiate receptor cDNA has been identified from a cerebral cortical CDNA library using sequences from the rat mu opiate receptor CDNA. The human mu opiate receptor (hmuOR1) shares 95% amino acid identity with the rat sequence. The expressed muOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat mu opiate receptor. Effects on cyclic AMP are similar to those noted for the rat mu opiate receptor. Overlapping genomic clones spanning 50 kilobasepairs and hybridizing with the hmuOR1 cDNA contains exon sequences encoding the entire open reading frame of the human A opiate receptor are described. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, is being used to assess this gene's involvement in neuropsychiatric disorders involving opiatergic systems.