RECONSTRUCTING ORGANS FROM DECELLULARIZED BIOMATERIAL SCAFFOLD

摘要

The invention is directed to compositions and methods for reconstructing an artificial organ. The artificial organ is reconstructed using a three-dimensional scaffold produced by decellularizing biostructures from a donor organ. The three-dimensional scaffold is perfused with endothelial cells that develop to produce an endothelial tissue layer with a primitive vascular system that sustains the growth and development of a second cultured cell population. When grown in the three-dimensional scaffold and endothelial tissue layer, with the primitive vascular system, the cells of the second cell population proliferate, mature and differentiate into neomorphic organ structures that are analogous to their in vivo counterparts.