T cell receptors (TCRs) that have higher affinity for ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
申请公布号
WO0148145(A2)
申请公布日期
2001.07.05
申请号
WO2000US42622
申请日期
2000.12.06
申请人
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人
KRANZ, DAVID, M.;WITTRUP, K., DANE;HOLLER, PHILLIP, D.