| 摘要 |
PURPOSE: A medium including human serum for human cell cultivation is provided to eliminate existing problems such as the delay of multiplication speed and the loss of phenotype. CONSTITUTION: The medium for human cell cultivation includes 1-50%(v/v), preferably 5-20%(v/v), of human serum. Other ingredients of the medium are insulin, transferrin, selenium, T3 hormone (3,3,5-triiodo-L-thyronine), transforming growth factor (TGF-β), insulin-like growth factor (IGF), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), keratinocyte growth factor (KGF), and epidermal growth factor (EGF). Each 1mg/l-15mg/l of insulin and transferrin is used while 1μg/l-15μg/l of T3 hormone (3,3,5-triiodo-L-thyronine) used.
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