| 摘要 |
The present invention relates to a novel method for enrichment of specific nucleic acid segments, such as a DNA, e.g. single nucleotide polymorphisms (SNPs), sequences that have been deleted, sequences that are identical between two complex genomes, etc. The present method includes steps for providing a first sample A and a second sample B derived from different sources and digestion of both said samples; amplification of sample A with a suitable primer and dNTPs comprising one unconventional base and amplification of sample B with a labelled primer and all the conventional dNTPs, followed by combination of samples A and B; denaturation and hybridization; treatment with a nuclease specific for said unconventional base, such as uracil-DNA glycosylase (UDG), and isolation of the specific segment originally present in sample B by use of the primer label. In a second aspect, the present invention relates to a kit which comprises components suitable for working the above described method. |
