| 摘要 |
A method for obtaining high yield, high purity taxane analogues from a source containing a mixture of taxanes. The method employs a polymeric resin membrane for separating the analogues under low pressure without the use of complex and expensive separation/purification steps currently provided in the art. Specifically, the method involves: extracting a source of taxanes in an organic extractant; coating a weak absorbent material with the extractant and loading the medium in a column coated with an absorbent agent; eluting, in a first step, with an organic solvent mixture at a pressure of between 10 and 20 psi to generate fractions containing taxane compounds; crystallizing the fractions to provide a solid taxane compound and a mother liquor; concentrating the mother liquor; and eluting, in a second step, with a polar solvent mixture the mother liquor through a polymeric resin in a chromatography column to provide at least a second taxane compound.
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