| 摘要 |
An in vitro method for the determination of specific dopamine-bets-hydroxylase activity for the evaluation of the sympathetic nervous system function of a patient from a serum sample comprising the steps of: a) filtering the serum sample to substantially remove drugs and endogenous inhibitors of dopamine-beta-hydroxylase, b) incubating the filtered sample of step a) with a substrate of dopamine-beta-hydroxylase for conversion into a detectable compound, c) determining the amount of detectable compound to evaluate dopamine-beta-hydroxylase activity and comparing with a normal sample, whereby an amount lower than a normal sample is indicative of a reduced sympathetic nervous system function and an amount higher than a normal sample is indicative of an increased sympathetic nervous system function. A reduction in the sympathetic nervous system function is indicative of neurodegenerative, neuroendocrine, psycho-affective and cardiovascular diseases, burnout, and chronic fatigue, and the effects of stress such as panic syndrome. An increased in the sympathetic nervous system function is indicative of the effects of stress.
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