An ex vivo animal or challenge model as method to measure protective immunity directed against parasites and vaccines shown to be protective in said method

摘要

An ex vivo animal or challenge model as a method to identify protective (recombinant) proteins and rapidly measure protective immunity in intestinal segments, directed against parasites and vaccines directed against parasitic infections and vaccines directed against infection with parasites, such as Fasciola hepatica. The vaccines contain protective (recombinant) proteins identified and shown to be protective in studies using the ex vivo model. The protective (recombinant) proteins are derived from newly excysted juveniles (NEJ) of Fasciola hepatica, and the protective (recombinant) protein or fragments thereof have an apparent molecular weight of 32 kD and N-terminal amino acid sequence comprising the sequence XXDVSWPFWDRMYNY.