| 摘要 |
<p>This invention provides a bioluminescence resonance energy transfer (BRET) fusion molecule, and method of use. The fusion molecule comprises three components: a bioluminescent donor protein (BDP), a modulator, and a fluorescent acceptor molecule (FAM), wherein the FAM can accept energy from the BDP-generated luminescence when these components are in an appropriate spatial relationship and in the presence of an appropriate substrate. The modulator can either influence the proximity/orientation of the BDP and the FAM and thereby the energy transfer between these components, or it can play a different role in affecting the energy transfer between the BDP-generated activated product and the FAM.</p> |
