PROCESS FOR THE SCALEABLE PURIFICATION OF PLASMID DNA
摘要
The present invention relates to a nonchromatographic-based process for the isolation of clinical grade plasmid DNA from bacterial cells. The exemplified methods described herein outline a scaleable, economically favorable protocol for the purification of clinical grade plasmid DNA from E. coli which includes CTAB-based precipitation of DNA in combination with adsorption of impurities to calcium silicate.
申请公布号
WO0146215(A1)
申请公布日期
2001.06.28
申请号
WO2000US34725
申请日期
2000.12.21
申请人
MERCK & CO., INC.;LANDER, RUSSEL, J.;WINTERS, MICHAEL;MEACLE, FRANCIS
发明人
LANDER, RUSSEL, J.;WINTERS, MICHAEL;MEACLE, FRANCIS