Homogeneous and multiplex assays for target nucleic acids hybridized to oligonucleotide probes immobilized on particulate and non-particulate substrates are described. Target nucleic acids are determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle, or by spatial resolution of oligonucleotides immobilized at specific loci on a substrate. Target-correlated fluorescence signal, originating from a target nucleic acid hybridized to the substrate-immobilized oligonucleotide is determined as a measure of the presence or amount of the target nucleic acid.
申请公布号
WO0146474(A1)
申请公布日期
2001.06.28
申请号
WO2000US34726
申请日期
2000.12.21
申请人
ORTHO-CLINICAL DIAGNOSTICS, INC.;MERCOLINO, THOMAS, J.;SERINO, GERMAN;CONNELLY, MARK, C.
发明人
MERCOLINO, THOMAS, J.;SERINO, GERMAN;CONNELLY, MARK, C.